Recombinant Human PRIMPOL Protein, GST-tagged

• Cat.No.: PRIMPOL-5235H
• Product Overview: Human CCDC111 full-length ORF (BAB71553.1, 1 a.a. - 560 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a DNA primase-polymerase that belongs to a superfamily of archaeao-eukaryotic primases. Members of this family have primase activity, catalyzing the synthesis of short RNA primers that serve as starting points for DNA synthesis, as well as DNA polymerase activity. The encoded protein facilitates DNA damage tolerance by mediating uninterrupted fork progression after UV irradiation and reinitiating DNA synthesis. An allelic variant in this gene is associated with myopia 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 90.8 kDa
• AA Sequence: MNRKWEAKLKQIEERASHYERKPLS SVYRPRLSKPEEPPSIWRLFHRQAQ AFNFVKSCKEDVHVFALECKVGDGQ RIYLVTTYAEFWFYYKSRKNLLHCY EVIPENAVCKLYFDLEFNKPANPGA DGKKMVALLIEYVCKALQELYGVNC SAEDVLNLDSSTDEKFSRHLIFQLH DVAFKDNIHVGNFLRKILQPALDLL GSEDDDSAPETTGHGFPHFSEAPAR QGFSFNKMFTEKATEESWTSNSKKL ERLGSAEQSSPDLSFLVVKNNMGEK HLFVDLGVYTRNRNFRLYKSSKIGK RVALEVTEDNKFFPIQSKDVSDEYQ YFLSSLVSNVRFSDTLRILTCEPSQ NKQKGVGYFNSIGTSVETIEGFQCS PYPEVDHFVLSLVNKDGIKGGIRRW NYFFPEELLVYDICKYRWCENIGRA HKSNNIMILVDLKNEVWYQKCHDPV CKAENFKSDCFPLPAEVCLLFLFKE EEEFTTDEADETRSNETQNPHKPSP SRLSTGASADAVWDNGIDDAYFLEA TEDAELAEAAENSLLSYNSEVDEIP DELIIEVLQE
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: PRIMPOL primase and DNA directed polymerase [ Homo sapiens (human) ]
• Official Symbol: PRIMPOL
• Synonyms: CCDC111; PRIMPOL; primase and DNA directed polymerase; MYP22; Primpol1; DNA-directed primase/polymerase protein; coiled-coil domain-containing protein 111; hPrimpol1; EC 2.7.7.-
• Gene ID: 201973
• mRNA Refseq: NM_001300767
• Protein Refseq: NP_001287696
• MIM: 615421
• UniProt ID: Q96LW4