||Recombinant Human PRKAA1 protein, fused to MYC/DDK-tagged at C-terminus, was expressed in HEK293.
||The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
||25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
||> 80% as determined by SDS-PAGE and Coomassie blue staining
||>50 ug/mL as determined by microplate BCA method