Recombinant Human PRKCA Protein, GST-tagged

• Cat.No.: PRKCA-011H
• Product Overview: Active recombinant N-terminal GST-tagged full length human PKCα protein (1-672) was expressed in insect cells.

Specification

• Description: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
• Source: Insect cell
• Species: Human
• Tag: GST
• Form: Lyophilized
• Molecular Mass: 102 kDa
• Protein length: 1-672
• Purity: ≥80% as determined by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to produce 1 pmol of ADP per minuteat 30 centigrade in 0.1mM CaCl2, 1 mg/mL phosphatidylserine, 0.02 mg/mL diacylglycerol, and 0.3% Triton X-100 using histone H1 (0.1 mg/mL) and 20μM ATP as substrates. Signal read after a 40 min reactionvia luminescent detection.
• Stability: ≥ 2 years
• Storage: At -80 centigrade.
• Storage Buffer: Sterile 40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, 0.04% Tween 20, 3 mM DTT, 20% glycerol, and 1.6 mM glutathione

Gene Information

• Gene Name: PRKCA protein kinase C alpha [ Homo sapiens (human) ]
• Official Symbol: PRKCA
• Synonyms: PRKCA; protein kinase C alpha; AAG6; PKCA; PRKACA; PKCI+/-; PKCalpha; PKC-alpha; protein kinase C alpha type; PKC-A; aging-associated gene 6; EC 2.7.11.13
• Gene ID: 5578
• mRNA Refseq: NM_002737
• Protein Refseq: NP_002728
• MIM: 176960
• UniProt ID: P17252