"PRL" Related Products

Recombinant Human PRL Protein, MIgG2a Fc-tagged, low endotoxin

Cat.No. : PRL-384H
Product Overview : Recombinant Human PRL Protein, MIgG2a Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Cys 227 (Accession # Q5THQ0-1). Predicted N-terminus: Leu 29. This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 49.8 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Source : HEK293
Species : Human
Tag : MIgG2a Fc
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Recombinant Human PRL Protein, MIgG2a Fc-tagged, low endotoxin at 1 μg/mL (100 μL/well) can bind Human Prolactin R / PRLR Protein, Fc Tag with a linear range of 2-39 ng/mL (QC tested).
Molecular Mass : 49.8 kDa
Protein length : Leu 29 - Cys 227
Endotoxin : Less than 0.1 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Figure :

Gene Name : PRL prolactin [ Homo sapiens ]
Official Symbol : PRL
Synonyms : PRL; prolactin; decidual prolactin;
Gene ID : 5617
mRNA Refseq : NM_000948
Protein Refseq : NP_000939
MIM : 176760
UniProt ID : P01236

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