| Species : |
Human |
| Source : |
Human |
| Tag : |
His |
| Description : |
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides |
| Form : |
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50% glycerol, 0.2M NaCl |
| Molecular Weight : |
25.1 kDa (225aa), confirmed by MALDI-TOF |
| Purity : |
> 90% by SDS - PAGE |
| Concentration : |
0.25 mg/ml (determined by Bradford assay) |
| Sequences of amino acids : |
MGSSHHHHHH SSGLVPRGSH MSIMSYNGGA VMAMKGKNCV AIAADRRFGI QAQMVTTDFQ KIFPMGDRLY IGLAGLATDV QTVAQRLKFR LNLYELKEGR QIKPYTLMSM VANLLYEKRF GPYYTEPVIA GLDPKTFKPF ICSLDLIGCP MVTDDFVVSG TCAEQMYGMC ESLWEPNMDP DHLFETISQA MLNAVDRDAV SGMGVIVHII EKDKITTRTL KARMD |
| Storage : |
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
| Pathways : |
APC/C-mediated degradation of cell cycle proteins; APC/C:Cdc20 mediated degradation of Securin; Adaptive Immunity Signaling; Apoptosis; Assembly of the pre-replicative complex; Autodegradation of the E3 ubiquitin ligase COP1; CDK-mediated phosphorylation and removal of Cdc6; CDT1 association with the CDC6:ORC:origin complex; Cell Cycle Checkpoints; Cell Cycle, Mitotic; Cyclin A:Cdk2-associated events at S phase entry; Cyclin E associated events during G1/S transition; Vpu mediated degradation of CD4; Vif-mediated degradation of APOBEC3G; Synthesis of DNA |
