Recombinant Human PRPF4 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PRPF4-4811H
• Product Overview: PRPF4 MS Standard C13 and N15-labeled recombinant protein (NP_004688) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 58.4 kDa
• AA Sequence: MASSRASSTQATKTKAPDDLVAPVV KKPHIYYGSLEEKERERLAKGESGI LGKDGLKAGIEAGNINITSGEVFEI EEHISERQAEVLAEFERRKRARQIN VSTDDSEVKACLRALGEPITLFGEG PAERRERLRNILSVVGTDALKKTKK DDEKSKKSKEEYQQTWYHEGPNSLK VARLWIANYSLPRAMKRLEEARLHK EIPETTRTSQMQELHKSLRSLNNFC SQIGDDRPISYCHFSPNSKMLATAC WSGLCKLWSVPDCNLLHTLRGHNTN VGAIVFHPKSTVSLDPKDVNLASCA ADGSVKLWSLDSDEPVADIEGHTVR VARVMWHPSGRFLGTTCYDRSWRLW DLEAQEEILHQEGHSMGVYDIAFHQ DGSLAGTGGLDAFGRVWDLRTGRCI MFLEGHLKEIYGINFSPNGYHIATG SGDNTCKVWDLRQRRCVYTIPAHQN LVTGVKFEPIHGNFLLTGAYDNTAK IWTHPGWSPLKTLAGHEGKVMGLDI SSDGQLIATCSYDRTFKLWMAETRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PRPF4 pre-mRNA processing factor 4 [ Homo sapiens (human) ]
• Official Symbol: PRPF4
• Synonyms: PRPF4; PRP4 pre-mRNA processing factor 4 homolog (yeast); U4/U6 small nuclear ribonucleoprotein Prp4; HPRP4; HPRP4P; PRP4; PRP4/STK/WD splicing factor; Prp4p; PRP4 homolog; WD splicing factor Prp4; U4/U6 snRNP 60 kDa protein
• Gene ID: 9128
• mRNA Refseq: NM_004697
• Protein Refseq: NP_004688
• MIM: 607795
• UniProt ID: O43172