Recombinant Human PTGS2 protein, FLAG/His-tagged

• Cat.No.: PTGS2-32H
• Product Overview: Recombinant Human PTGS2(1-604 aa) fused with FLAG/His tag at C-terminal was expressed in Insect cells.

Specification

• Description: Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.
• Source: Insect cells
• Species: Human
• Tag: FLAG/His
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 µg/ml FLAG peptide, 300 µM diethyldithiocarbamate and 0.08% Triton X-100.
• Molecular Mass: 71 kDa
• AA Sequence: MLARALLLCAVLALSHTANPCCSHP CQNRGVCMSVGFDQYKCDCTRTGFY GENCSTPEFLTRIKLFLKPTPNTVH YILTHFKGFWNVVNNIPFLRNAIMS YVLTSRSHLIDSPPTYNADYGYKSW EAFSNLSYYTRALPPVPDDCPTPL GVKGKKQLPDSNEIVEKLLLRRKFI PDPQGSNMMFAFFAQHFTHQFFKTD HKRGPAFTNGLGHGVDLNHIYGETL ARQRKLRLFKDGKMKYQIIDGEMYP PTVKDTQAEMIYPPQVPEHLRFAVG QEVFGLVPGLMMYATIWLREHNRV CDVLKQEHPEWGDEQLFQTSRLILI GETIKIVIEDYVQHLSGYHFKLKFD PELLFNKQFQYQNRIAAEFNTLYH WHPLLPDTFQIHDQKYNYQQFIYNN SILLEHGITQFVESFTRQIAGRVAG GRNVPPAVQKVSQASIDQSRQMKYQ SFNEYRKRFMLKPYESFEELTGEKE MSAELEALYGDIDAVELYPALLVEK PRPDAIFGETMVEVGAPFSLKGLM GNVICSPAYWKPSTFGGEVGFQIIN TASIQSLICNNVKGCPFTSFSVPDP ELIKTVTINASSSRSGLDDINPTVL LKERSTELDYKDDDDKHHHHHH
• Purity: ≥90%
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: At least 6 months at -80 centigrade. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
• Concentration: 0.33 mg/ml

Gene Information

• Gene Name: PTGS2 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) [ Homo sapiens ]
• Official Symbol: PTGS2
• Synonyms: PTGS2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); prostaglandin G/H synthase 2; COX2; PHS II; PGH synthase 2; cyclooxygenase 2b; prostaglandin H2 synthase 2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS
• Gene ID: 5743
• mRNA Refseq: NM_000963
• Protein Refseq: NP_000954
• MIM: 600262
• UniProt ID: P35354
• Chromosome Location: 1q25.2-q25.3
• Pathway: Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Biological oxidations, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; COX reactions, organism-specific biosystem
• Function: enzyme binding; heme binding; heme binding; lipid binding; metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; peroxidase activity; peroxidase activity; peroxidase activity; prostaglandin-endoperoxide synthase activity

Reviews

01/30/2023

The staff was very nice, communicated with me positively and solved a lot of technical problems.

08/20/2022

The technician gave me a thorough introduction to the PTGS2 protein so that I could understand its biochemical properties.

04/07/2022

High quality product at a good price, a satisfactory shopping experience.

Q&As

What biological processes is PTGS2 involved in?

PTGS2 is involved in the inflammatory cascade and increases the response of inflammatory factors such as TNF-α, IL-1β, and IL-6.

What diseases has PTGS2 been closely associated with the study of?

PTGS2 has been closely associated with studies of glandular cystitis and cerebral small vessel disease.

What is the relationship between PTGS2 and BCL6?

BCL6 acts at the promoter of PTGS2.

Is PTGS2 involved in prostaglandin synthesis?

PTGS2 is a key gene for prostaglandin synthesis in pathological conditions.

Where is PTGS2 localized?

PTGS2 is localized in the nuclear membrane.

How is PTGS2 expressed in glandular cystitis?

PTGS2 expression showed high levels in glandular cystitis and its involvement in the development of glandular cystitis.