Recombinant Human PUS3 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human PUS3 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : PUS3-4633H
Product Overview : PUS3 MS Standard C13 and N15-labeled recombinant protein (NP_112597) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : The protein encoded by this gene catalyzes the formation of tRNA pseudouridine from tRNA uridine at position 39 in the anticodon stem and loop of transfer RNAs. Two transcript variants encoding different isoforms have been found for this gene.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 55.6 kDa
AA Sequence : MADNDTDRNQ TEKLLKRVRE LEQEVQRLKK EQAKNKEDSN IRENSSGAGK TKRAFDFSAH GRRHVALRIA YMGWGYQGFA SQENTNNTIE EKLFEALTKT RLVESRQTSN YHRCGRTDKG VSAFGQVISL DLRSQFPRGR DSEDFNVKEE ANAAAEEIRY THILNRVLPP DIRILAWAPV EPSFSARFSC LERTYRYFFP RADLDIVTMD YAAQKYVGTH DFRNLCKMDV ANGVINFQRT ILSAQVQLVG QSPGEGRWQE PFQLCQFEVT GQAFLYHQVR CMMAILFLIG QGMEKPEIID ELLNIEKNPQ KPQYSMAVEF PLVLYDCKFE NVKWIYDQEA QEFNITHLQQ LWANHAVKTH MLYSMLQGLD TVPVPCGIGP KMDGMTEWGN VKPSVIKQTS AFVEGVKMRT YKPLMDRPKC QGLESRIQHF VRRGRIEHPH LFHEEETKAK RDCNDTLEED NTNLETPTKR VCVDTEIKSI ITRTRPLEQK LISEEDLAAN DILDYKDDDD KV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : PUS3 pseudouridylate synthase 3 [ Homo sapiens (human) ]
Official Symbol : PUS3
Synonyms : PUS3; pseudouridylate synthase 3; MRT55; FKSG32; NEDMIGS; 2610020J05Rik; tRNA pseudouridine(38/39) synthase; pseudouridylate synthase 3; tRNA pseudouridine synthase 3; tRNA pseudouridylate synthase 3; tRNA-uridine isomerase 3; EC 5.4.99.45
Gene ID : 83480
mRNA Refseq : NM_031307
Protein Refseq : NP_112597
MIM : 616283
UniProt ID : Q9BZE2

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