Active Recombinant Human PVRIG protein, His-tagged

• Cat.No.: PVRIG-565H
• Product Overview: Recombinant Human PVRIG protein(Thr 41 - Asp 171), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Thr 41 - Asp 171
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Human Nectin-2, Fc Tag at 5 μg/mL (100 μL/well) can bind Human PVRIG, His Tag with a linear range of 0.08-2.5 μg/mL. Immobilized Biotinylated Human Nectin-2, Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Human PVRIG Protein, His Tag with a linear range of 10-78 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 15.7 kDa. The protein migrates as 22-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PVRIG poliovirus receptor related immunoglobulin domain containing [ Homo sapiens ]
• Official Symbol: PVRIG
• Synonyms: PVRIG; poliovirus receptor related immunoglobulin domain containing; transmembrane protein PVRIG; C7orf15; MGC2463; poliovirus receptor-related immunoglobulin domain-containing protein; MGC104322; MGC138295; MGC138297
• Gene ID: 79037
• mRNA Refseq: NM_024070
• Protein Refseq: NP_076975
• UniProt ID: Q6DKI7