Recombinant Human PVRIG protein, His-tagged

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Recombinant Human PVRIG protein, His-tagged

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Cat.No. : PVRIG-565H
Product Overview : Recombinant Human PVRIG (Thr41-Asp171) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family. The CD112R gene encodes a putative single transmembrane protein, which is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. Notably, the intracellular domain of phatases. The extracellular domain sequence of human and mouse CD112R have 65.3% similarity. CD112R may act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Thr41
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 15.6 kDa. The protein migrates as 23-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Thr41-Asp171
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of PVRIG-565H :

Human PVRIG, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of PVRIG-565H :

Immobilized Human PVRIG, His Tag at 2 μg/mL (100 μL/well) can bind Human Nectin-2, Fc Tag with a linear range of 0.5-8 ng/mL (QC tested).

Bioactivity-ELISA :

Immobilized Biotinylated Human Nectin-2, Fc,Avitag at 1 μg/mL (100 μL/well) can bind Human PVRIG, His Tag with a linear range of 1-39 ng/mL (Routinely tested).

Gene Name : PVRIG
Official Symbol : PVRIG
Synonyms : PVRIG; poliovirus receptor related immunoglobulin domain containing; transmembrane protein PVRIG; C7orf15; MGC2463; poliovirus receptor-related immunoglobulin domain-containing protein; MGC104322; MGC138295; MGC138297
Gene ID : 79037
mRNA Refseq : NM_024070
Protein Refseq : NP_076975
MIM : 617012
UniProt ID : Q6DKI7

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