Recombinant Human RAB27A, His-tagged

• Cat.No.: RAB27A-2479H
• Product Overview: Recombinant human RAB27A, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 27 kDa (241aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Ras-related protein Rab-27A, also known as RAB27A, belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Also, RAB27A regulates the cytotoxic granule exocytosis and affect T-lymphocyte and macrophage-activation. It is widely expressed with significant expression in pancreatic islets and pituitary tissue, and low expression in brain. Defects in RAB27A cause Griscelli syndrome type 2 (GS2).
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol.
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MSDGDYDYLI KFLALGDSGV GKTSVLYQYT DGKFNSKFIT TVGIDFREKR VVYRASGPDG ATGRGQRIHL QLWDTAGQER FRSLTTAFFR DAMGFLLLFD LTNEQSFLNV RNWISQLQMH AYCENPDIVL CGNKSDLEDQ RVVKEEEAIA LAEKYGIPYF ETSAANGTNI SQAIEMLLDL IMKRMERCVD KSWIPEGVVR SNGHASTDQL SEEKEKGACG C.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: RAB27A RAB27A, member RAS oncogene family [ Homo sapiens ]
• Synonyms: RAB27A, member RAS oncogene family; GS2; RAM; RAB27; HsT18676; MGC117246; RAB27A; ras-related protein Rab-27A; rab-27; GTP-binding protein Ram;Ras-related protein Rab-27A; Rab-27
• Gene ID: 5873
• mRNA Refseq: NM_004580
• Protein Refseq: NP_004571
• MIM: 603868
• UniProt ID: P51159
• Chromosome Location: 15q15-q21.1
• Pathway: Diabetes pathways
• Function: GTP binding; GTPase activity; myosin V binding; nucleotide binding; protein binding