Recombinant Human RAF1 Protein, His-tagged

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Recombinant Human RAF1 Protein, His-tagged

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Cat.No. : RAF1-442H
Product Overview : Recombinant human RAF1 protein, fused to His tag, was expressed in Sf9 cells.
Description : This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008].
Source : Insect cells
Species : Human
Tag : His
Form : 50 mM Tris.HCl pH 8.0, 150 mM NaCl, 20 % glycerol. Store at -80 centigrade. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
Molecular Mass : 39 kDa
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration : >50 ug/mL as determined by microplate BCA method
Gene Name : Raf-1 proto-oncogene, serine/threonine kinase [ Homo sapiens ]
Official Symbol : RAF1
Synonyms : c-Raf; CMD1NN; CRAF; NS5; Raf-1
Gene ID : 5894
mRNA Refseq : NM_002880.3
Protein Refseq : NP_002871.1
MIM : 164760
UniProt ID : P04049

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