Description : |
RAN is a small GTPase involved in the assembly of microtubules to form mitotic spindles. The protein encoded by this gene is a cytoplasmic guanine nucleotide exchange factor (GEF) that binds beta-tubulin and has GEF activity toward RAN. The encoded protein plays a role in the formation of noncentrosomal microtubules. In addition, this protein may be involved in the regulation of D(1) receptor signaling by protein kinase C delta and protein kinase C gamma. Several transcript variants encoding different isoforms have been found for this gene. |
Source : |
HEK293 |
Species : |
Human |
Tag : |
Myc/DDK |
Molecular Mass : |
67.2 kDa |
AA Sequence : |
MAAATADPGAGNPQPGDSSGGGAGG GLPSPGEQELSRRLQRLYPAVNQQE TPLPRSWSPKDKYNYIGLSQGNLRV HYKGHGKNHKDAASVRATHPIPAAC GIYYFEVKIVSKGRDGYMGIGLSAQ GVNMNRLPGWDKHSYGYHGDDGHSF CSSGTGQPYGPTFTTGDVIGCCVNL INGTCFYTKNGHSLGIAFTDLPANL YPTVGLQTPGEIVDANFGQQPFPFD IEDYMREWRAKVQGTVHCFPISARL GEWQAVLQNMVSSYLVHHGYCATAT AFARMTETPIQEEQASIKNRQKIQK LVLEGRVGEAIETTQRFYPGLLEHN PNLLFMLKCRQFVEMVNGTDSEVRS LSSRSPKSQDSYPGSPSLSPRHGPS SSHMHNTGADSPSCSNGVASTKSKQ NHSKYPAPSSSSSSSSSSSSSSPSS VNYSESNSTDSTKSQHHSSTSNQET SDSEVEMEAEHYPNGVLGSMSTRIV NGAYKHEDLQTDESSMDDRHPRRQL CGGNQAATERIILFGRELQALSEQL GREYGKNLAHTEMLQDAFSLLAYSD PWSCPVGQQLDPIQREPVCAALNSA ILESQNLPKQPPLMLALGQASECLR LMARAGLGSCSFARVDDYLHTRTRP LEQKLISEEDLAANDILDYKDDDDK V |
Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : |
50 μg/mL as determined by BCA |
Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |