Recombinant Human RBM25 protein, GST-tagged

• Cat.No.: RBM25-2212H
• Product Overview: Recombinant RBM25 protein, fused to GST-tag, was expressed in E.coli.

Specification

• Description: RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. Also involved in the generation of an abnormal and truncated splice form of SCN5A in heart failure.
• Source: E.coli
• Species: Human
• Tag: GST
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.). 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Molecular Mass: 52 kDa
• Protein length: 235
• AA Sequence: LKPTLRPISSAPSVSSASGNATPNT PGDESPCGIIIPHENSPDQQQPEEH RPKIGLSLKLGASNSPGQPNSVKRK KLPVDSVFNKFEDEDSDDVPRKRKL VPLDYGEDDKNATKGTVNTEEKRKH IKSLIEKIPTAKPELFAYPLDWSIV DSILMERRIRPWINKKIIEYIGEEE ATLVDFVCSKVMAHSSPQSILDDVA MVLDEEAEVFIVKMWRLLIYETEAK KIGLVK
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8 centigrade for (1-2 weeks).
  Long-term storage: Aliquot and store at -20 centigrade to -80 centigrade for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.
  If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).

Gene Information

• Gene Name: RBM25
• Official Symbol: RBM25
• Synonyms: S164; NET52; RNPC7; Snu71; RED120; fSAP94
• Gene ID: 58517
• mRNA Refseq: NM_021239.3
• Protein Refseq: NP_067062.1
• MIM: 612427
• UniProt ID: P49756