Active Recombinant Human RGMA protein, His-Avi-tagged, Low Endotoxin

• Cat.No.: RGMA-5744H
• Product Overview: Recombinant Human RGMA protein(Q96B86-1)(48-422 aa), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 48-422 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human RGMa, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 44.5 kDa. Due to autocatalytic cleavage, the protein migrates to 27-35 kDa and 40-50 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: RGMA RGM domain family, member A [ Homo sapiens ]
• Official Symbol: RGMA
• Synonyms: RGMA; RGM domain family, member A; repulsive guidance molecule A; RGM
• Gene ID: 56963
• mRNA Refseq: NM_001166283
• Protein Refseq: NP_001159755
• MIM: 607362
• UniProt ID: Q96B86