Recombinant Human RGS10, His-tagged

• Cat.No.: RGS10-3599H
• Product Overview: Recombinant human RGS10 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-181 aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 10(RGS10) belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. This protein associates specifically with the activated forms of the two related G-protein subunits, G-alphai3 and G-alphaz but fails to interact with the structurally and functionally distinct G-alpha subunits. RGS10 protein is localized in the nucleus.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
• Molecular Weight: 23.7 kDa (205aa), confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMFNRAV SRLSRKRPPS DIHDSDGSSS SSHQSLKSTA KWAASLENLL EDPEGVKRFR EFLKKEFSEE NVLFWLACED FKKMQDKTQM QEKAKEIYMT FLSSKASSQV NVEGQSRLNE KILEEPHPLM FQKLQDQIFN LMKYDSYSRF LKSDLFLKHK RTEEEEEDLP DAQTAAKRAS RIYNT
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: RGS10 regulator of G-protein signaling 10 [ Homo sapiens ]
• Official Symbol: RGS10
• Synonyms: regulator of G-protein signaling 10; RGS10; regulator of G-protein signaling 10; OTTHUMP00000020597; OTTHUMP00000020598
• Gene ID: 6001
• mRNA Refseq: NM_001005339
• Protein Refseq: NP_001005339
• MIM: 602856
• UniProt ID: O43665
• Chromosome Location: 10q25
• Function: GTPase activator activity; protein binding; signal transducer activity