| Description : |
This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
61.4 kDa |
| AA Sequence : |
MPGKPKHLGVPNGRMVLAVSDGELS STTGPQGQGEGRGSSLSIHSLPSGP SSPFPTEEQPVASWALSFERLLQDP LGLAYFTEFLKKEFSAENVTFWKAC ERFQQIPASDTQQLAQEARNIYQEF LSSQALSPVNIDRQAWLGEEVLAEP RPDMFRAQQLQIFNLMKFDSYARFV KSPLYRECLLAEAEGRPLREPGSSR LGSPDATRKKPKLKPGKSLPLGVEE LGQLPPVEGPGGRPLRKSFRRELGG TANAALRRESQGSLNSSASLDLGFL AFVSSKSESHRKSLGSTEGESESRP GKYCCVYLPDGTASLALARPGLTIR DMLAGICEKRGLSLPDIKVYLVGNE QALVLDQDCTVLADQEVRLENRITF ELELTALERVVRISAKPTKRLQEAL QPILEKHGLSPLEVVLHRPGEKQPL DLGKLVSSVAAQRLVLDTLPGVKIS KARDKSPCRSQGCPPRTQDKATHPP PASPSSLVKVPSSATGKRQTCDIEG LVELLNRVQSSGAHDQRGLLRKEDL VLPEFLQLPAQGPSSEETPPQTKSA AQPIGGSLNSTTDSALTRTRPLEQK LISEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
