Recombinant Human RHEB Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: RHEB-5588H
• Product Overview: RHEB MS Standard C13 and N15-labeled recombinant protein (NP_005605) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.
• Molecular Mass: 20.3 kDa
• AA Sequence: MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: RHEB Ras homolog enriched in brain [ Homo sapiens (human) ]
• Official Symbol: RHEB
• Synonyms: RHEB; Ras homolog enriched in brain; Ras homolog enriched in brain 2, RHEB2; GTP-binding protein Rheb; Ras homolog enriched in brain 2; RHEB2; MGC111559
• Gene ID: 6009
• mRNA Refseq: NM_005614
• Protein Refseq: NP_005605
• MIM: 601293
• UniProt ID: Q15382