Recombinant Human RHOA protein, His-tagged

• Cat.No.: RHOA-32H
• Product Overview: Recombinant Human RHOA fused with histidine tag in the amino-terminus was expressed in E. coli. The constitutively active form of human RhoA protein contains a glutamine to leucine substitution at residue 63. The common name for this mutant is RhoA(Q63L) or L63RhoA. The leucine substitution prevents endogenous and GAP-stimulated GTPase activity of RhoA, hence the protein is always in the active, GTP-bound, state. The corresponding mutation in Ras (Ras(Q61L)) behaves as an oncogenic mutation.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The protein is supplied as a lyophilized powder. When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose and 0.1% dextran.
• Molecular Mass: The recombinant protein is 25 kDa, consisting of the 22 kDa RhoA constitutively active protein plus a histidine tag in the amino-terminus.
• Purity: 90%
• Applications: RhoA effector and GAP identification and binding studies;
  Activation of RhoA effectors in vitro and in vivo;
  Stimulation of RhoA phenotype by microinjection into cells.

Gene Information

• Gene Name: RHOA ras homolog family member A [ Homo sapiens ]
• Official Symbol: RHOA
• Synonyms: RHOA; ras homolog family member A; ARH12, ARHA, ras homolog gene family, member A; transforming protein RhoA; Rho12; RhoA; RHOH12; h12; oncogene RHO H12; rho cDNA clone 12; Aplysia ras-related homolog 12; small GTP binding protein RhoA; ras homolog gene family, member A; ARHA; ARH12; RHO12
• Gene ID: 387
• mRNA Refseq: NM_001664
• Protein Refseq: NP_001655
• MIM: 165390
• UniProt ID: P61586
• Chromosome Location: 3p21.3
• Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Arf6 downstream pathway, organism-specific biosystem; Aurora B signaling, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem
• Function: GTP binding; GTPase activity; myosin binding; nucleotide binding; protein binding