| Description : |
Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
60.2 kDa |
| AA Sequence : |
MEPRAVAEAVETGEEDVIMEALRSY NQEHSQSFTFDDAQQEDRKRLAELL VSVLEQGLPPSHRVIWLQSVRILSR DRNCLDPFTSRQSLQALACYADISV SEGSVPESADMDVVLESLKCLCNLV LSSPVAQMLAAEARLVVKLTERVGL YRERSFPHDVQFFDLRLLFLLTALR TDVRQQLFQELKGVRLLTDTLELTL GVTPEGNPPPTLLPSQETERAMEIL KVLFNITLDSIKGEVDEEDAALYRH LGTLLRHCVMIATAGDRTEEFHGHA VNLLGNLPLKCLDVLLTLEPHGDST EFMGVNMDVIRALLIFLEKRLHKTH RLKESVAPVLSVLTECARMHRPARK FLKAQGWPPPQVLPPLRDVRTRPEV GEMLRNKLVRLMTHLDTDVKRVAAE FLFVLCSESVPRFIKYTGYGNAAGL LAARGLMAGGRPEGQYSEDEDTDTD EYKEAKASINPVTGRVEEKPPNPME GMTEEQKEHEAMKLVTMFDKLSRNR VIQPMGMSPRGHLTSLQDAMCETME QQLSSDPDSDPDTRTRPLEQKLISE EDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
