"RNF43" Related Products

Recombinant Human RNF43 Protein, Fc-tagged

Cat.No.: RNF43-767H
Product Overview: Recombinant human RNF43 (NP_060233.3) (Met1-Tyr197) was expressed with the Fc region of human IgG1 at the C-terminus.
Source: HEK293
Species: Human
Tag: Fc
Predicted N Terminal: Gly 24
Form: Lyophilized from sterile PBS, pH 7.4, 5% ~ 8% trehalose and mannitol.
Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human RSPO3-His (146) at 10 μg/mL (100 μL/well) can bind human RNF43-Fc. The EC50 of human RNF43-Fc is 0.01-0.03μg/mL.
Molecular Mass: The recombinant human RNF43/Fc comprises 412 amino acids and has a predicted molecular mass of 45.8 kDa. The apparent molecular mass of the protein is approximately 52 kDa in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: RNF43 ring finger protein 43 [ Homo sapiens ]
Official Symbol: RNF43
Synonyms: URCC; RNF124
Gene ID: 54894
mRNA Refseq: NM_017763.4
Protein Refseq: NP_060233.3
MIM: 612482
UniProt ID: Q68DV7
Chromosome Location: 17q22

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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