Recombinant Human ROBO4 protein, His-tagged

• Cat.No.: ROBO4-3792H
• Product Overview: Recombinant Human ROBO4 protein(Q8WZ75-1)(Ala27-Glu469), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Ala27-Glu469
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human ROBO4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: ROBO4 roundabout, axon guidance receptor, homolog 4 (Drosophila) [ Homo sapiens ]
• Official Symbol: ROBO4
• Synonyms: ROBO4; roundabout, axon guidance receptor, homolog 4 (Drosophila); roundabout homolog 4 (Drosophila); roundabout homolog 4; ECSM4; FLJ20798; magic roundabout; MRB; roundabout homolog 4, magic roundabout; MGC133352; MGC133353
• Gene ID: 54538
• mRNA Refseq: NM_019055
• Protein Refseq: NP_061928
• MIM: 607528
• UniProt ID: Q8WZ75