Recombinant Mouse ROBO4 Protein, His-tagged

• Cat.No.: ROBO4-957M
• Product Overview: Recombinant mouse ROBO4 (NP_083059.2) N-terminal fragment (Met 1-Glu 480) was expressed, with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-480 a.a.
• Predicted N Terminal: Leu 38
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Molecular Mass: The secreted recombinant mouse ROBO4 (aa 1-480) comprises 454 amino acids and has a calculated molecular mass of 49.6 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 65 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >97 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Robo4 roundabout homolog 4 (Drosophila) [ Mus musculus ]
• Official Symbol: ROBO4
• Synonyms: ROBO4; roundabout homolog 4 (Drosophila); roundabout homolog 4; Magic roundabout; AI593217; 1200012D01Rik
• Gene ID: 74144
• mRNA Refseq: NM_028783
• Protein Refseq: NP_083059
• Function: protein binding; receptor activity