Recombinant Human ROCK2 Protein, GST-Tagged

• Cat.No.: ROCK2-337H
• Product Overview: Recombinant Human ROCK2 protein(NP_001308572.1)(1-181 aa), fused to GST-tag at N-terminus, was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.). 5 % trehalose and 5 % mannitol are added as protectants before lyophilization.
• Bio-activity: Not tested.
• Protein length: 1-181 aa
• AA Sequence: MEIDMTYQLKVIQQSLEQEEAEHKA TKARLADKNKIYESIEEAKSEAMKE MEKKLLEERTLKQKVENLLLEAEKR CSLLDCDLKQSQQKINELLKQKDVL NEDVRNLTLKIEQETQKRCLTQNDL KMQTQQVNTLKMSEKQLKQENNHLM EMKMNLEKQNAELRKERQDADGQMK ELQDQL
• Endotoxin: Please contact the lab for more information.
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Stability: Store for up to 12 months at -20°C to -80°C as lyophilized powder.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
  If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
  Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.

Gene Information

• Gene Name: ROCK2 Rho associated coiled-coil containing protein kinase 2 [ Homo sapiens (human) ]
• Official Symbol: ROCK2
• Synonyms: KIAA0619
• Gene ID: 9475
• mRNA Refseq: NM_001321643.2
• Protein Refseq: NP_001308572.1
• MIM: 604002
• UniProt ID: O75116

Reviews

08/19/2021

Swift and hassle-free delivery ensured our experiments stayed on track.

01/13/2020

Efficient customer service provided valuable insights for our decision-making.

07/06/2019

Reliable and precise—this recombined protein never disappoints.

Q&As

Discuss the role of ROCK2 in smooth muscle contraction and vascular tone.

ROCK2 is a key regulator of smooth muscle contraction, influencing vascular tone by phosphorylating myosin light chain and promoting actin-myosin interaction.

Describe the molecular crosstalk between ROCK2 and other signaling pathways.

ROCK2 interacts with various signaling pathways, including Wnt, PI3K/Akt, and MAPK pathways, forming a network that regulates cell survival, proliferation, and differentiation.

How does ROCK2 contribute to the regulation of cell cycle progression?

ROCK2 can influence the cell cycle by regulating cyclin-dependent kinase activity and promoting the phosphorylation of proteins involved in cell cycle checkpoints.

What are the implications of ROCK2 in cell migration and invasion?

ROCK2 is involved in the regulation of cell migration and invasion by controlling actin dynamics and focal adhesion turnover, influencing processes like metastasis in cancer.

Discuss the role of ROCK2 in the regulation of the actin cytoskeleton.

ROCK2 phosphorylates various downstream targets, such as myosin light chain, leading to actin-myosin contraction and the reorganization of the actin cytoskeleton.