Recombinant Human RP1 Protein, His-tagged

• Cat.No.: RP1-2659H
• Product Overview: Recombinant Human RP1 Protein (Lys36-Asp233) with a N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes a member of the doublecortin family. The protein encoded by this gene contains two doublecortin domains, which bind microtubules and regulate microtubule polymerization. The encoded protein is a photoreceptor microtubule-associated protein and is required for correct stacking of outer segment disc. This protein and the RP1L1 protein, another retinal-specific protein, play essential and synergistic roles in affecting photosensitivity and outer segment morphogenesis of rod photoreceptors. Because of its response to in vivo retinal oxygen levels, this protein was initially named ORP1 (oxygen-regulated protein-1). This protein was subsequently designated RP1 (retinitis pigmentosa 1) when it was found that mutations in this gene cause autosomal dominant retinitis pigmentosa. Mutations in this gene also cause autosomal recessive retinitis pigmentosa. Transcript variants resulted from an alternative promoter and alternative splicings have been found, which overlap the current reference sequence and has several exons upstream and downstream of the current reference sequence. However, the biological validity and full-length nature of some variants cannot be determined at this time.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 25.8 kDa
  Accurate Molecular Mass: 31 kDa
• Protein length: Lys36-Asp233
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: RP1 RP1 axonemal microtubule associated [ Homo sapiens (human) ]
• Official Symbol: RP1
• Synonyms: RP1; RP1 axonemal microtubule associated; ORP1; DCDC4A; oxygen-regulated protein 1
  doublecortin domain containing 4A
  retinitis pigmentosa 1 (autosomal dominant)
  retinitis pigmentosa 1 protein
  retinitis pigmentosa RP1 protein
• Gene ID: 6101
• mRNA Refseq: NM_006269
• Protein Refseq: NP_006260
• MIM: 603937
• UniProt ID: P56715