Recombinant Human RPN1 Protein, His&GST-tagged

• Cat.No.: RPN1-2033H
• Product Overview: Recombinant Human RPN1 Protein (Ser272-Lys517) with N-His&GST tag was expressed in E. coli.

Specification

• Description: This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.
• Source: E. coli
• Species: Human
• Tag: N-His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 58.3 kDa
  Accurate Molecular Mass: 58 kDa
• Protein length: Ser272-Lys517
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: RPN1 ribophorin I [ Homo sapiens (human) ]
• Official Symbol: RPN1
• Synonyms: RPN1; ribophorin I; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; oligosaccharyltransferase 1 homolog (S. cerevisiae); OST1; RPN-I; ribophorin-1; oligosaccharyltransferase 1 homolog; dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit; dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; RBPH1; DKFZp686B16177
• Gene ID: 6184
• mRNA Refseq: NM_002950
• Protein Refseq: NP_002941
• MIM: 180470
• UniProt ID: P04843