Active Recombinant Human RSPO1 Protein, His-tagged

• Cat.No.: RSPO1-3872H
• Product Overview: Active Recombinant Human RSPO1 Protein(Met 1-Ala 146)(NP_001033722.1) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-146 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to bind recombinant mouse CD36 in a functional ELISA.
• Molecular Mass: The secreted recombinant human RSPO1 (aa 1-146) comprises 137 amino acids and has a predicted molecular mass of 14 kDa. As a result of glycosylation, rhRSPO1 migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_001033722.1
• Unit ID: Q2MKA7
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/Q2MKA7

Gene Information

• Gene Name: RSPO1 R-spondin 1 [ Homo sapiens ]
• Official Symbol: RSPO1
• Synonyms: RSPO1; R-spondin 1; R spondin homolog (Xenopus laevis); R-spondin-1; FLJ40906; RSPONDIN; R-spondin homolog; roof plate-specific spondin-1; RSPO; CRISTIN3; RP11-566C13.1
• Gene ID: 284654
• mRNA Refseq: NM_001038633
• protein Refseq: NP_001033722
• MIM: 609595