| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
1-263 aa |
| Form : |
Lyophilized from sterile PBS, pH 7.4. |
| Bio-activity : |
1. Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind human LIMPII with a linear ranger of 32-800 ng/ml.
2. Measured by its binding ability in a functio+A1nal ELISA. Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind mouse CD36 with a linear ranger of 6.4-800 ng/ml.
3. Measured by its ability to induce activation of response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.05-0.5 μg/mL in the presence of 5 ng/mL recombinant mouse Wnt3a. |
| Molecular Mass : |
The secreted recombinant human RSPO1 comprises 254 amino acids with a predicted molecular mass of 28.2 kDa. As a result of glycosylation, rhRSPO1 migrates as an approximately 42 kDa band in SDS-PAGE under reducing conditions. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity : |
> 98 % as determined by SDS-PAGE |
| Storage : |
Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| protein Refseq-Weblink : |
http://www.ncbi.nlm.nih.gov/protein/NP_001033722.1 |
| Unit ID : |
Q2MKA7 |
| Unit ID-Weblink : |
https://www.uniprot.org/uniprotkb/Q2MKA7 |
