Recombinant Human S100B protein, His-tagged

• Cat.No.: S100B-7016H
• Product Overview: Recombinant Human S100B (Met1-Glu92) protein was fused to His-tag at N-terminus and expressed in E.coli.

Specification

• Description: S-100 protein beta chain (S100B),a member of the S-100 family, is also known as S-100 protein subunit beta and S100 calcium-binding protein B, which contains two EF-hand domains. S100B can weakly bind calcium but bind zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Also, S100B can bind to and initiate the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction,S100B may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Fuethermore,S100B could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. S100B may mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.
• Source: E.coli
• Species: Human
• Tag: His
• Predicted N Terminal: Met1
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 8.0, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 11.5 kDa. The protein migrates as 11 kDa under reducing (R) condition (SDS-PAGE).
• Protein length: Met1-Glu92
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: S100B
• Official Symbol: S100B
• Synonyms: S100B; S100 calcium binding protein B; S100 calcium binding protein, beta (neural); protein S100-B; S100beta; S-100 protein subunit beta; S-100 calcium-binding protein, beta chain; S100 calcium-binding protein, beta (neural); NEF; S100; S100-B
• Gene ID: 6285
• mRNA Refseq: NM_006272
• Protein Refseq: NP_006263
• MIM: 176990
• UniProt ID: P04271

Reviews

03/15/2022

One major advantage of S100B protein is its ability to contribute to the understanding of diseases related to aberrant cell cycle regulation, including cancer.

09/29/2019

The S100B protein demonstrates exceptional quality, making it an ideal candidate to fulfill my experimental requirements.

10/23/2018

The manufacturer of S100B protein plays a crucial role in supporting my research efforts.

Q&As

How does S100B help in the diagnosis of traumatic brain injury (TBI)?

Elevated S100B levels in the blood can indicate TBI, and its measurement can aid in TBI diagnosis and severity assessment.

What conditions can S100B be used to diagnose?

S100B can be used to diagnose brain injuries, such as traumatic brain injury and stroke.

How is S100B measured in clinical settings?

S100B levels are typically measured through a blood test.

What precautions should be taken when interpreting S100B results?

Results should be interpreted in the context of the patient's clinical history and other diagnostic tests.

How is S100B used in the context of neurodegenerative diseases?

S100B may help in monitoring disease progression and severity in conditions like Alzheimer's disease.