Recombinant Human Selenoprotein X, 1, His-tagged

• Cat.No.: SEPX1-2827H
• Product Overview: Recombinant human SEPX1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. MW = 14.8 kDa(136aa), confirmed by MALDI-TOF.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: SEPX1, also known as Methionine sulfoxide reductase B1 (MSRB1), is a selenoprotein. Methionine sulfoxide reductases(MSRs) catalyze reduction of free and protein-bound methionine sulfoxides to corresponding methionines. The oxidation of methionine by ROS generates a diastereomeric mixture of methionine-S-sulfoxide (Met-S-SO) and methionine-R-sulfoxide (Met-R-SO). Two distinct enzyme families evolved for reduction of these sulfoxides, with methionine-S-sulfoxide reductase (MsrA) being stereospecific for Met-S-SO and methionine-R-sulfoxide reductase (MsrB) for Met-R-SO.In bacteria, the selenocystein (Sec/U) element is located immediately following the UGA codon within the reading frame for the selenoprotein so we mutated Sec-95 to Cys.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MSFCSFFGGE VFQNHFEPGV YVCAKCGYEL FSSRSKYAHS SPWPAFTETI HADSVAKRPE HNRSEALKVS CGKCGNGLGH EFLNDGPKPG QSRFCIFSSS LKFVPKGKET SASQGH
• Purity: > 90% by SDS – PAGE.
• Form: Liquid. In 20 mM Tris-HCl Buffer (pH 7.5) containing 1 mM DTT, 0.1 mM PMSF, 2mM EDTA, 10% Glycerol.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: SEPX1 selenoprotein X, 1 [ Homo sapiens ]
• Synonyms: SEPX1; selenoprotein X, 1; SELR; SELX; MSRB1; HSPC270; MGC3344; methionine-R-sulfoxide reductase B1; selenoprotein R; methionine sulfoxide reductase; EC 1.8.4.-; Selenoprotein X
• Gene ID: 51734
• mRNA Refseq: NM_016332
• Protein Refseq: NP_057416
• MIM: 606216
• UniProt ID: Q9NZV6
• Chromosome Location: 16p13.3
• Function: metal ion binding; oxidoreductase activity; peptide-methionine-(S)-S-oxide reductase activity; zinc ion binding