Active Recombinant Human SEMA4A protein, His-tagged

• Cat.No.: SEMA4A-3926H
• Product Overview: Active Recombinant Human SEMA4A protein(NP_071762.2) extracellular domain (Met 1-His 683) was expressed in HEK293, with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-683 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to bind biotinylated mouse SEMA4D in a functional ELISA.
• Molecular Mass: The recombinant human SEMA4A consists of 663 amino acids and predictes a molecular mass of 73.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh SEMA4A is approximately 75-80 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SEMA4A sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A [ Homo sapiens ]
• Official Symbol: SEMA4A
• Synonyms: SEMA4A; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A; SEMAB; semaphorin-4A; FLJ12287; SemB; sema B; semaphorin-B; RP35; SEMB; CORD10; RP11-54H19.2
• Gene ID: 64218
• mRNA Refseq: NM_001193300
• Protein Refseq: NP_001180229
• MIM: 607292
• UniProt ID: Q9H3S1