"SEMA4D" Related Products

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Recombinant Human SEMA4D protein, Fc-tagged

Cat.No.: SEMA4D-513H
Product Overview: Recombinant Human SEMA4D(Met 22 - Arg 734) fused with a human IgG1 Fc tag at the C-terminus was expressed in HEK293.
Source: HEK293
Species: Human
Tag: Fc
Predicted N Terminal: Met 22
Form: Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Bio-activity: Measured by its ability to promote the survival and differentiation of human peripheral blood monocytes.The ED50 for this effect is typically 2.0-6.3 μg/mL.
Molecular Mass: Has a calculated MW of 105.4 kDa. DTT-reduced Protein migrates as 120-140 kDa in SDS-PAGE due to glycosylation.
Protein length: Met 22 - Arg 734
Endotoxin: Less than 1.0 EU per μg of the Human Semaphorin 4D, Fc Tag by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
Gene Name: SEMA4D sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D [ Homo sapiens ]
Official Symbol: SEMA4D
Synonyms: SEMA4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; C9orf164, chromosome 9 open reading frame 164 , SEMAJ; semaphorin-4D; CD100; coll 4; FLJ39737; M sema G; A8; GR3; BB18; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D; SEMAJ; coll-4; C9orf164; M-sema-G; FLJ33485; FLJ34282; FLJ46484; MGC169138; MGC169141;
Gene ID: 10507
mRNA Refseq: NM_001142287
Protein Refseq: NP_001135759
MIM: 601866
UniProt ID: Q92854
Chromosome Location: 9q22-q31
Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Sema4D in semaphorin signaling, organism-specific biosystem; Sema4D induced cell migration and growth-cone collapse, organism-specific biosystem; Sema4D mediated inhibition of cell attachment and migration, organism-specific biosystem;
Function: protein binding; receptor activity; receptor binding; semaphorin receptor binding; transmembrane signaling receptor activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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