Recombinant Human SENP1 Catalytic Domain, His-tagged

• Cat.No.: SENP1-688H
• Product Overview: Recombinant, human SENP1 Catalytic Domain consistingof amino acids 415-643 was fused with a His Tag.

Specification

• Species: Human
• Tag: His
• Protein Length: 415-643 a.a.
• Description: The covalent modification of proteins by SUMO is reversibleand is mediated by SENP enzymes. SENPs cleave residues from the C-terminus ofSUMO precursor proteins to generate the mature and active form which containsthe conserved C-terminal di-glycine. These enzymes also reverse the processof SUMOylation by removing poly-SUMO conjugates from substrate proteins.SENP1 is active against SUMO-1, SUMO-2 and SUMO-3 in vitro but not ubiquitinor NEDD8.
• MolecularWeight: 29.8 kDa
• Stock: In 50 mM Hepes pH 8.0, 100 mM NaCl, 10% glycerol, 5 mM DTT.
• Purity: > 95% bySDS-PAGE
• Use: Typical enzymeconcentration to support hydrolysis of substrates in vitro is 50-500 nM depending onconditions. Pre-incubation (15 min) with 10 mM DTT isrecommended toachieve maximum activity
• Storage: Store at-80°C. Avoid multiple freeze/thaw cycles.

Gene Information

• Gene Name: SENP1SUMO1/sentrin specific peptidase 1 [ Homo sapiens ]
• Official Symbol: SENP1
• Synonyms: SENP1; SUMO1/sentrin specific peptidase 1; SuPr-2;sentrin-specific protease 1; SUMO1/sentrin specific protease 1;sentrin/SUMO-specific protease SENP1
• Gene ID: 29843
• mRNA Refseq: NM_014554
• Protein Refseq: NP_055369
• MIM: 612157
• UniProt ID: Q9P0U3
• Chromosome Location: 12q13.1
• Pathway: Androgen Receptor Signaling Pathway; Regulationof Androgen receptor activity
• Function: cysteine-typepeptidase activity; endopeptidase activity; peptidase activity