Recombinant Human SENP1, His-tagged

• Cat.No.: SENP1-687H
• Product Overview: Recombinant, human SENP1 consisting of aminoacids 419-643 fused at the N-terminus to a His Tag and expressed in E.coli.

Specification

• Cat. No.: SENP1-687H
• Description: SENP1, a nuclear SUMO protease, regulatesandrogen receptor transactivation by targeting histone deacetylase 1 andc-Jun activity through deSUMOylation of p300. Recent data shows SENP1 isrequired for HIF-dependent transcription during embryogenesis and in responseto hypoxia. The protease is useful in the study of SENP1 endopeptidase andisopeptidase activity and for inhibitor screening.
• Source: E. Coli
• Formulation: In 50 mM HEPES, 5% glycerol pH 7.4.
• Biologicalactivity: EC50:≥1.42 nM as determined using proSUMO-1 substrate in an endopeptidase assay.
• Purity: ≥90% by SDS-PAGE
• Applications: Enzyme-linkedImmunoabsorbent Assay; Western Blot (Recombinant protein); AntibodyProduction;ProteinArray
• Storagebuffer: 50mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
• Storage: ≤-70°C

Gene Information

• Gene Name: SENP1SUMO1/sentrin specific peptidase 1 [ Homo sapiens ]
• Official Symbol: SENP1
• Synonyms: SENP1; SUMO1/sentrin specific peptidase 1; SuPr-2;sentrin-specific protease 1; SUMO1/sentrin specific protease 1;sentrin/SUMO-specific protease SENP1
• Gene ID: 29843
• mRNA Refseq: NM_014554
• Protein Refseq: NP_055369
• MIM: 612157
• UniProt ID: Q9P0U3
• Chromosome Location: 12q13.1
• Pathway: Androgen Receptor Signaling Pathway; Regulationof Androgen receptor activity
• Function: cysteine-typepeptidase activity; endopeptidase activity; peptidase activity