Recombinant Human Serine/Threonine Kinase 10, His-tagged

• Cat.No.: STK10-555H
• Product Overview: Recombinant human LOK was expressed by baculovirus inSf9 insect cellsusing an N-terminal His tag. MW=43.2 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: LOK is a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. LOK can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. LOK can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. MEKK1 and LOK have opposing roles in regulating the enhancer element of the IL-2 gene during T-cell activation. While MEKK1 is responsible for the CD28 signalling pathway that activates the CD28 response element, co-expression of LOK attenuates these effects.
• Purity: 60%.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, and 1 mM DTT.
• Stability: >6 months at –80°C.

Gene Information

• Gene Name: STK10 serine/threonine kinase 10 [ Homo sapiens ]
• Synonyms: STK10; serine/threonine kinase 10; LOK; PRO2729; lymphocyte-oriented kinase; EC 2.7.11.1
• Gene ID: 6793
• mRNA Refseq: NM_005990
• Protein Refseq: NP_005981
• MIM: 603919
• UniProt ID: O94804
• Chromosome Location: 5q35.1
• Function: ATP binding; nucleotide binding; protein serine/threonine kinase activity; transferase activity