Recombinant Human SIGLEC15 protein, Fc-tagged
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|Product Overview :||Recombinant Human SIGLEC15(Phe20-Thr263) fused with Fc of Human IgG1 tag at C-terminal was expressed in CHO.|
|Description :||Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Within the ECD, human Siglec-15 shares 85% aa sequence identity with mouse and rat Siglec-15. Alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10. It is expressed on osteoclasts, macrophages, and dendritic cells and binds to the sialyl-Tn antigen. This interaction induces the production of TGF-beta by tumor-associated macrophages. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.|
|Predicted N Terminal :||Phe20|
|Form :||Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, CHAPS and Trehalose.|
|Bio-activity :||Measured by its binding ability in a functional ELISA. When Neu5Ac(a2-6)GalNAc-PAA-biotin is immobilized onto a Streptavidin-coated plate, Recombinant Human Siglec-15 Fc Chimera binds with an ED50 of 150-900 ng/mL.|
|Molecular Mass :||
Predicted Molecular Mass: 53 kDa;
SDS-PAGE: 53-64 kDa, reducing conditions
|Protein length :||Phe20-Thr263|
|Endotoxin :||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity :||>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.|
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution :||Reconstitute at 200 μg/mL in water.|
|Shipping :||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Gene Name :||SIGLEC15 sialic acid binding Ig like lectin 15 [ Homo sapiens ]|
|Official Symbol :||SIGLEC15|
|Synonyms :||CD33L3; HsT1361; SIGLEC-15; CD33 antigen-like 3; CD33 molecule-like 3|
|Gene ID :||284266|
|mRNA Refseq :||NM_213602|
|Protein Refseq :||NP_998767|
|UniProt ID :||Q6ZMC9|
|Chromosome Location :||18q12.3|
|Pathway :||DAP12 interactions, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem|
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