Recombinant Human SIGLEC8 protein, His-Avi-tagged, Biotinylated

• Cat.No.: SIGLEC8-197HB
• Product Overview: Biotinylated Recombinant human siglec 8 Protein is expressed from mammalian with a His Tag and Avi Tag at the C-terminal. It contains Met17-Ala363.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: It contains Met17-Ala363.
• Description: Siglec-8, also known as SAF, is an approximately 75 kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains.Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid.
• Form: Supplied as 0.22um filtered solution in 20mM PBS,pH7.4.
• Molecular Mass: The protein has a predicted MW of 40.7 kDa.Due to glycosylation, the protein migrates to 50-60KDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.05 EU per ug by the LAL method.
• Purity: > 95% as determined by Tris-Bis -PAGE; > 95% as determined by HPLC
• Storage: The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: SIGLEC8 sialic acid binding Ig-like lectin 8 [ Homo sapiens ]
• Official Symbol: SIGLEC8
• Synonyms: SAF2; SIGLEC-8; SIGLEC8L
• Gene ID: 27181
• mRNA Refseq: NM_014442
• Protein Refseq: NP_055257
• MIM: 605639
• UniProt ID: Q9NYZ4