Recombinant Human SIGLEC8 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: SIGLEC8-1014H
• Product Overview: SIGLEC8 MS Standard C13 and N15-labeled recombinant protein (NP_055257) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 ), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM, and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM) that mediates an association with SLAM-associated protein.
• Molecular Mass: 54.1 kDa
• AA Sequence: MLLLLLLLPLLWGTKGMEGDRQYGDGYLLQVQELVTVQEGLCVHVPCSFSYPQDGWTDSDPVHGYWFRAGDRPYQDAPVATNNPDREVQAETQGRFQLLGDIWSNDCSLSIRDARKRDKGSYFFRLERGSMKWSYKSQLNYKTKQLSVFVTALTHRPDILILGTLESGHPRNLTCSVPWACKQGTPPMISWIGASVSSPGPTTARSSVLTLTPKPQDHGTSLTCQVTLPGTGVTTTSTVRLDVSYPPWNLTMTVFQGDATASTALGNGSSLSVLEGQSLRLVCAVNSNPPARLSWTRGSLTLCPSRSSNPGLLELPRVHVRDEGEFTCRAQNAQGSQHISLSLSLQNEGTGTSRPVSQVTLAAVGGAGATALAFLSFCIIFIIVRSCRKKSARPAAGVGDTGMEDAKAIRGSASQGPLTESWKDGNPLKKPPPAVAPSSGEEGELHYATLSFHKVKPQDPQGQEATDSEYSEIKIHKRETAETQACLRNHNPSSKEVRGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: SIGLEC8 sialic acid binding Ig-like lectin 8 [ Homo sapiens (human) ]
• Official Symbol: SIGLEC8
• Synonyms: SIGLEC8; sialic acid binding Ig-like lectin 8; sialic acid-binding Ig-like lectin 8; MGC59785; SAF2; SIGLEC 8; SIGLEC8L; SAF-2; CDw329; sialoadhesin family member 2; SIGLEC-8
• Gene ID: 27181
• mRNA Refseq: NM_014442
• Protein Refseq: NP_055257
• MIM: 605639
• UniProt ID: Q9NYZ4