Recombinant Human SIGLEC8 protein, His & GST-tagged SIGLEC8-1799H

Recombinant Human SIGLEC8 protein, His & GST-tagged

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Recombinant Human SIGLEC8 protein, His & GST-tagged

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Cat.No. : SIGLEC8-1799H
Product Overview : Recombinant Human SIGLEC8 aa. (Gly70~Ser336 (Accession # Q9NYZ4)) fused with N-terminal His & GST tag was produced in E. coli cells.
Description : Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).
Source : E. coli
Species : Human
Tag : His & GST
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 59.0kDa.
Protein length : Gly70~Ser336 (Accession # Q9NYZ4)
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 9.2.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage Buffer : Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Reconstitution : Reconstitute in sterile ddH2O.
Figure :

SDS-PAGE

Gene Name : SIGLEC8 sialic acid binding Ig like lectin 8 [ Homo sapiens (human) ]
Official Symbol : SIGLEC8
Synonyms : Fbln5; fibulin 5; A55; EVEC; DANCE; fibulin-5; FIBL-5; developmental arteries and neural crest EGF-like protein
Gene ID : 27181
mRNA Refseq : NM_014442.2
Protein Refseq : NP_055257.2
UniProt ID : Q9NYZ4

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