|Product Overview :
||Human Sirtuin 6 (GenBank Accession No. NM_016539), full length with N-terminal GST-tag was expressed in an E. coli expression system.
||This gene encodes a member of the sirtuin family of NAD-dependent enzymes that are implicated in cellular stress resistance, genomic stability, aging and energy homeostasis. The encoded protein is localized to the nucleus, exhibits ADP-ribosyl transferase and histone deacetylase activities, and plays a role in DNA repair, maintenance of telomeric chromatin, inflammation, lipid and glucose metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms.
||Aqueous buffer solution
Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS SIRT6 substrate 2, 500 µM NAD+ and Sirtuin 6 (18 – 2000 ng). Incubation condition: 60 min at 37ºC followed by developing for 15 min at room temperature.
|Molecular Mass :
||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
||At least 6 months at –80 centigrade. Avoid freeze/thaw cycles.
|Storage Buffer :
||40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol
||Avoid freeze/thaw cycles.