Recombinant Human SIRT6 Protein, GST-tagged

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Recombinant Human SIRT6 Protein, GST-tagged

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Cat.No. : SIRT6-28517H
Product Overview : Human Sirtuin 6 (GenBank Accession No. NM_016539), full length with N-terminal GST-tag was expressed in an E. coli expression system.
Description : This gene encodes a member of the sirtuin family of NAD-dependent enzymes that are implicated in cellular stress resistance, genomic stability, aging and energy homeostasis. The encoded protein is localized to the nucleus, exhibits ADP-ribosyl transferase and histone deacetylase activities, and plays a role in DNA repair, maintenance of telomeric chromatin, inflammation, lipid and glucose metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms.
Source : E.coli
Species : Human
Tag : GST
Form : Aqueous buffer solution
Bio-activity : 1.5 pmol/min/µg
Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS SIRT6 substrate 2, 500 µM NAD+ and Sirtuin 6 (18 – 2000 ng). Incubation condition: 60 min at 37ºC followed by developing for 15 min at room temperature.
Molecular Mass : 65 kDa
Applications : Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage : At least 6 months at –80 centigrade. Avoid freeze/thaw cycles.
Storage Buffer : 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol
Warning : Avoid freeze/thaw cycles.
Gene Name : SIRT6 sirtuin 6 [ Homo sapiens (human) ]
Official Symbol : SIRT6
Synonyms : SIRT6; sirtuin 6; SIR2L6; NAD-dependent protein deacetylase sirtuin-6; SIR2-like protein 6; regulatory protein SIR2 homolog 6; sir2-related protein type 6; sirtuin type 6; EC 2.4.2.31
Gene ID : 51548
mRNA Refseq : NM_016539
Protein Refseq : NP_057623
MIM : 606211
UniProt ID : Q8N6T7

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