Recombinant Human SLAMF7 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: SLAMF7-186HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human SLAMF7/CD319 /CRACC Protein, C-Fc Tag (rh SLAMF7/CD319 Fc Chimera) Ser 23-Met 226 (Accession # AAH27867) was produced in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Description: SLAM family member 7 (SLAMF7) is also known as CD2-like receptor-activating cytotoxic cells (CRACC), Membrane protein FOAP-12, CD antigen CD319, Novel Ly9, Protein 19A, which is a single-pass type I membrane protein and a member of the CD2 family of cell surface receptors. SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Isoform 1 of SLAMF7 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway. May play a role in lymphocyte adhesion. Isoform 3 of SLAMF7 does not mediate any NK cell activation.
• Form: Lyophilized
• Molecular Mass: rh SLAMF7/CD319 Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 49 kDa. DTT-reduced Protein migrates as 60-67 kDa in SDS-PAGE due to glycosylation.
• N-terminal Sequence Analysis: Ser 23
• Endotoxin: < 1.0 EU/ μg of the rh SLAMF7/CD319 Fc Chimera by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Conjugation: Alexa Fluor 555

Gene Information

• Gene Name: SLAMF7 SLAM family member 7 [ Homo sapiens ]
• Official Symbol: SLAMF7
• Synonyms: SLAMF7; SLAM family member 7; 19A; CD319; CRACC; CS1; protein 19A; CD2 subset 1; 19A24 protein; membrane protein FOAP-12; CD2-like receptor activating cytotoxic cells; CD2-like receptor-activating cytotoxic cells; novel LY9 (lymphocyte antigen 9) like protein
• Gene ID: 57823
• mRNA Refseq: NM_021181
• Protein Refseq: NP_067004
• MIM: 606625
• UniProt ID: Q9NQ25