Recombinant Human SMAD6 protein, His-tagged

• Cat.No.: SMAD6-7874H
• Product Overview: Recombinant Human SMAD6 protein(30-180 aa), fused with N-terminal His tag, was expressed in E. coli.

Specification

• Species: Human
• Source: E. coli
• Tag: His
• Protein Length: 30-180 aa
• Form: The purified protein was lyophilized from sterile phosphate-buffered saline (PBS: 58 mM Na₂HPO₄, 17 mM NaH₂PO₄, 68 mM NaCl, pH 7.4). Prior to lyophilization, 5% (w/v) trehalose and 5% (w/v) mannitol were incorporated as cryoprotective excipients. The protein was eluted with a buffer containing 300 mM imidazole.
• AASequence: GGGGDEDGSLGSRAEPAPRAREGGGCGRSEVRPVAPRRPRDAVGQRGAQGAGRRRRAGGPPRPMSEPGAGAGSSLLDVAEPGGPGWLPESDCETVTCCLFSERDAAGAPRDASDPLAGAALEPAGGGRSREARSRLLLLEQELKTVTYSLL
• Purity: 85%, by SDS-PAGE.
• Storage: Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SMAD6 SMAD family member 6 [ Homo sapiens ]
• Official Symbol: SMAD6
• Synonyms: SMAD6; SMAD family member 6; MAD, mothers against decapentaplegic homolog 6 (Drosophila) , MADH6, MADH7, SMAD, mothers against DPP homolog 6 (Drosophila); mothers against decapentaplegic homolog 6; HsT17432; SMAD 6; hSMAD6; MAD homolog 6; mothers against DPP homolog 6; SMAD, mothers against DPP homolog 6; Mothers against decapentaplegic, drosophila, homolog of, 6; MADH6; MADH7
• mRNA Refseq: NM_001142861
• Protein Refseq: NP_001136333
• MIM: 602931
• UniProt ID: O43541
• Gene ID: 4091