Recombinant Human SMO overexpression lysate

• Cat.No.: SMO-1617HCL
• Product Overview: Transient overexpression lysate of smoothened homolog (Drosophila) (SMO) was abtained from Human HEK293T cell line.

Specification

• Source: HEK293T
• Species: Human
• Tag: Myc/DDK
• Form: RIPA buffer
• Molecular Mass: 86.2 kDa
• Usage: 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
  1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
  1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
• Stability: Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles.
• Storage: Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20 centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: SMO smoothened, frizzled family receptor [ Homo sapiens ]
• Official Symbol: SMO
• Synonyms: SMO; smoothened, frizzled family receptor; SMOH, smoothened (Drosophila) homolog , smoothened homolog (Drosophila) , smoothened, seven transmembrane spanning receptor; smoothened homolog; frizzled family member 11; FZD11; protein Gx; seven transmembrane helix receptor; smoothened, seven transmembrane spanning receptor; Gx; SMOH
• Gene ID: 6608
• mRNA Refseq: NM_005631
• Protein Refseq: NP_005622
• MIM: 601500
• UniProt ID: Q99835