Recombinant Human SMURF2, FLAG-tagged

• Cat.No.: SMURF2-468H
• Product Overview: Recombinant Human SMURF2 (amino-acids 130-end) was expressed in a Baculovirus infected Sf9 cell expression system with N-terminal FLAG tag, MW= 72 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: Flag
• Protein Length: 130-end a.a.
• Description: SMURF2 has been shown to interact with Mothers against decapentaplegic homolog 7, SCYE1, SMURF1, Mothers against decapentaplegic homolog 3, Ubiquitin C, Mothers against decapentaplegic homolog 1 and Mothers against decapentaplegic homolog 2.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol, and 3 mM DTT.
• Application: Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Purity: > 80%.
• Stability: >6 months at -80°C.
• Publications:
  KRAS Protein Stability Is Regulated through SMURF2: UBCH5 Complex-Mediated β-TrCP1 Degradation1,2 (2014)
  Mutant EGFR is a preferred SMURF2 substrate of ubiquitination: role in enhanced receptor stability and TKI sensitivity (2020)

Gene Information

• Gene Name: SMURF2 SMAD specific E3 ubiquitin protein ligase 2 [ Homo sapiens ]
• Synonyms: SMAD specific E3 ubiquitin protein ligase 2; MGC138150; DKFZp686F0270; SMURF2; E3 ubiquitin ligase SMURF2; EC 6.3.2.-; hSMURF2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2
• Gene ID: 64750
• mRNA Refseq: NM_022739
• Protein Refseq: NP_073576
• MIM: 605532
• UniProt ID: Q9HAU4
• Chromosome Location: 17q22-q23
• Pathway: Endocytosis; TGF-beta signaling pathway; Ubiquitin mediated proteolysis; Signaling by BMP; Signaling by TGF beta
• Function: acid-amino acid ligase activity; identical protein binding; ligase activity; ubiquitin-protein ligase activity