Recombinant Human Soluble Tissue Factor

• Cat.No.: F3-28H
• Product Overview: Recombinant Human Soluble Tissue Factor produced inE. colihas a molecular mass of 25.624 Da.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Tissue factor is a cell-surface protein of endothelial cells and monocytes involved in coagulation and inflammation. It is the primary cellular initiator of coagulation cascade, and functions as receptor and cofactor for coagulation Factor VII and VIIa. Soluble human tissue factor with a histidine-tagged extracelullar domain is expressed inE. coli.
• Purity: ≥95% as determined by SDS-PAGE.
• Component: Tissue factor, PBS buffer, pH 7.4
• PI: 5.16 by Isoelectricfocusing
• concentration: 2 mg / ml by BCA
• Specific Activity: The activity is similar as that of full-length tissue factor using clotting assay.
• Endotoxin: less than 10EU/mg as determined by LAL method
• Storage Buffer: Liquid. In PBS Buffer.
• Storagee: At 4°C for 6 months and -80℃ for 2 years
• process: blood coagulation, blood coagulation, extrinsic pathway
• Publications:
  Thrombin generation response of rFVIIa in haemophilia A patient plasma when using different triggers and phospholipids. (2017)
  Determination of FVIIa-sTF Inhibitors in Toxic Microcystis Cyanobacteria by LC-MS Technique. (2015)
  Direct inhibition of factor VIIa by TFPI and TFPI constructs (2013)

Gene Information

• Gene Name: F3
• Synonyms: CD142; TF; TFAD; TFA; Thromboplastin; CD142 antigen; Coagulation factor III; Tissue factor precursor; coagulation factor III (thromboplastin, tissue factor); tissue factor,TF_HUMAN; F3
• Gene ID: 2152
• mRNA Refseq: NM_001993
• Protein Refseq: NP_001984
• MIM: 134390
• UniProt ID: P13726
• Chromosome Location: 1p22-p21

Citation

Determination of FVIIa-sTF Inhibitors in Toxic Microcystis Cyanobacteria by LC-MS Technique

Journal: Marine Drugs    PubMed ID: 26729138    Data: 2015/12/30

Authors: Andrea Roxanne J. Anas, Anna Nakajima, Vitor H. Pomin

Article Snippet:The sTF or Recombinant Human Soluble Tissue Factor (F3-28H) or Human F3 was purchased from Creative BioMart, Shirley, NY, USA.. The sTF was added with 10 mM PBS, pH 7.4, to make 1 mM (25.624 μg/mL), and transferred in 300 μL volumes in plastic cryogenic vials (Iwaki: 2712-002, Tokyo, Japan), stored at ?80 °C until use.The sTF was added with 10 mM PBS, pH 7.4, to make 1 mM (25.624 μg/mL), and transferred in 300 μL volumes in plastic cryogenic vials (Iwaki: 2712-002, Tokyo, Japan), stored at ?80 °C until use.

Direct inhibition of factor VIIa by TFPI and TFPI constructs.

Journal: Journal of thrombosis and haemostasis : JTH    PubMed ID: 23347185    Data: 2013/9/30

Authors: S Peraramelli, S Thomassen, M Dockal

Article Snippet:FVIIa was purchased from Novo Nordisk (Bagsvaerd, Denmark).FVIIa was purchased from Novo Nordisk (Bagsvaerd, Denmark).. Recombinant Soluble TF (sTF) was purchased from Creative BioMart (New York, NY, USA).. Human FXa (FXa) was purchased from Kordia (Leiden, the Netherlands) and bovine FXa (bFXa) was purified from bovine plasma [22].Human FXa (FXa) was purchased from Kordia (Leiden, the Netherlands) and bovine FXa (bFXa) was purified from bovine plasma [22].