Active Recombinant Human SOST protein, hFc-tagged, Low Endotoxin

• Cat.No.: SOST-4106H
• Product Overview: Recombinant Human SOST protein(Q9BQB4-1)(24-213 aa), fused with N-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Protein Length: 24-213 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human SOST, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 8.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 47.05 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: SOST sclerostin [ Homo sapiens ]
• Official Symbol: SOST
• Synonyms: SOST; sclerostin; sclerosteosis; VBCH
• Gene ID: 50964
• mRNA Refseq: NM_025237
• Protein Refseq: NP_079513
• MIM: 605740
• UniProt ID: Q9BQB4