Recombinant Human SPHK1 protein, His-tagged

Cat.No. : SPHK1-1299H
Product Overview : Recombinant Human SPHK1 aa. (Leu148~Leu398 (Accession # Q9NYA1) fused with N-terminal His tag was produced in E. coli cells.
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Description : The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms.
Source : E. coli
Species : Human
Tag : His
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 29.5kDa.
Protein length : Leu148~Leu398 (Accession # Q9NYA1)
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 6.9.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
SDS-PAGE:
Gene Name : SPHK1 sphingosine kinase 1 [ Homo sapiens (human) ]
Official Symbol : SPHK1
Synonyms : SPHK1; sphingosine kinase 1; SPHK; SK 1; SPK 1
Gene ID : 8877
mRNA Refseq : NM_001142601.1
Protein Refseq : NP_001136073.1
UniProt ID : Q9NYA1

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does SPHK1 contribute to drug resistance in cancer? 10/09/2021

Elevated SPHK1 levels have been associated with drug resistance in cancer cells, making it a potential target to overcome resistance mechanisms.

How does SPHK1 contribute to immune regulation? 08/03/2021

SPHK1 regulates immune cell trafficking and function by modulating S1P levels. This is significant in autoimmune diseases and inflammatory responses.

How might the modulation of SPHK1 impact personalized medicine? 04/06/2018

Understanding the role of SPHK1 in different diseases may contribute to the development of personalized therapeutic approaches, tailoring treatments based on individual patient characteristics and disease profiles.

How does SPHK1 influence vascular functions? 01/11/2017

SPHK1 is involved in endothelial cell functions and angiogenesis, making it a potential target in the treatment of vascular diseases.

Can SPHK1 inhibitors be used in combination therapies? 04/24/2016

Yes, combining SPHK1 inhibitors with other therapeutic agents is being explored to enhance treatment efficacy, especially in cancer.

Customer Reviews (3)

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Reviews
04/16/2023

    Its seamless integration makes it possible to explore different research avenues, expanding the scope and impact of my investigations.

    04/14/2020

      With the manufacturer's excellent technical support and its wide range of applications, the SPHK1 protein has become an indispensable tool, aiding in my research endeavors and driving meaningful scientific discoveries.

      01/03/2020

        Their team of experts is readily available to address any issues that may arise, providing solutions to problems and guiding me through the experimental process.

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