Recombinant Human SPHK1 Protein, N-His-tagged

• Cat.No.: SPHK1-12H
• Product Overview: Active recombinant human SPHK1 protein with N-terminal His-tag was expressed in Sf9 cells.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Molecular Mass: 47.5 kDa
• Unit Definition: One unit is defined as the amount of enzyme required to produce 1 nmol of ADP per minute at 25 centigrade in 20 mM Hepes, pH, 7.4, containing 50 mM NaCl, 10 mM MgCl2, 1 mM EGTA and 0.02% Triton X-100 and 50 μM Sphingosine (d18:1).
• Stability: ≥ 1 year
• Storage: At -80 centigrade
• Storage Buffer: 25 mM HEPES, pH 8.0, with 150 mM sodium chloride, 3 mM DTT, 0.05% polysorbate 20, and 25% glycerol
• Shipping: Dry ice in continental US; may vary elsewhere

Gene Information

• Gene Name: SPHK1 sphingosine kinase 1 [ Homo sapiens (human) ]
• Official Symbol: SPHK1
• Synonyms: SPHK1; sphingosine kinase 1; SPHK; sphingosine kinase 1; SK 1; SPK 1; acetyltransferase SPHK1; EC 2.7.1.91
• Gene ID: 8877
• mRNA Refseq: NM_182965
• Protein Refseq: NP_892010
• MIM: 603730
• UniProt ID: Q9NYA1