Recombinant Human SPHK1 Protein, N-His-tagged

• Cat.No.: SPHK1-12H
• Product Overview: Active recombinant human SPHK1 protein with N-terminal His-tag was expressed in Sf9 cells.

Specification

• Description: The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: Sf9 cells
• Species: Human
• Tag: His
• Molecular Mass: 47.5 kDa
• Unit Definition: One unit is defined as the amount of enzyme required to produce 1 nmol of ADP per minute at 25 centigrade in 20 mM Hepes, pH, 7.4, containing 50 mM NaCl, 10 mM MgCl2, 1 mM EGTA and 0.02% Triton X-100 and 50 μM Sphingosine (d18:1).
• Stability: ≥ 1 year
• Storage: At -80 centigrade
• Storage Buffer: 25 mM HEPES, pH 8.0, with 150 mM sodium chloride, 3 mM DTT, 0.05% polysorbate 20, and 25% glycerol
• Shipping: Dry ice in continental US; may vary elsewhere

Gene Information

• Gene Name: SPHK1 sphingosine kinase 1 [ Homo sapiens (human) ]
• Official Symbol: SPHK1
• Synonyms: SPHK1; sphingosine kinase 1; SPHK; sphingosine kinase 1; SK 1; SPK 1; acetyltransferase SPHK1; EC 2.7.1.91
• Gene ID: 8877
• mRNA Refseq: NM_182965
• Protein Refseq: NP_892010
• MIM: 603730
• UniProt ID: Q9NYA1

Reviews

04/16/2023

Its seamless integration makes it possible to explore different research avenues, expanding the scope and impact of my investigations.

04/14/2020

With the manufacturer's excellent technical support and its wide range of applications, the SPHK1 protein has become an indispensable tool, aiding in my research endeavors and driving meaningful scientific discoveries.

01/03/2020

Their team of experts is readily available to address any issues that may arise, providing solutions to problems and guiding me through the experimental process.

Q&As

How does SPHK1 contribute to drug resistance in cancer?

Elevated SPHK1 levels have been associated with drug resistance in cancer cells, making it a potential target to overcome resistance mechanisms.

How does SPHK1 contribute to immune regulation?

SPHK1 regulates immune cell trafficking and function by modulating S1P levels. This is significant in autoimmune diseases and inflammatory responses.

How might the modulation of SPHK1 impact personalized medicine?

Understanding the role of SPHK1 in different diseases may contribute to the development of personalized therapeutic approaches, tailoring treatments based on individual patient characteristics and disease profiles.

How does SPHK1 influence vascular functions?

SPHK1 is involved in endothelial cell functions and angiogenesis, making it a potential target in the treatment of vascular diseases.

Can SPHK1 inhibitors be used in combination therapies?

Yes, combining SPHK1 inhibitors with other therapeutic agents is being explored to enhance treatment efficacy, especially in cancer.