Recombinant Human SPP1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human SPP1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : SPP1-3044H
Product Overview : SPP1 MS Standard C13 and N15-labeled recombinant protein (NP_001035149) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 32.4 kDa
AA Sequence : MRIAVICFCL LGITCAIPVK QADSGSSEEK QNAVSSEETN DFKQETLPSK SNESHDHMDD MDDEDDDDHV DSQDSIDSND SDDVDDTDDS HQSDESHHSD ESDELVTDFP TDLPATEVFT PVVPTVDTYD GRGDSVVYGL RSKSKKFRRP DIQYPDATDE DITSHMESEE LNGAYKAIPV AQDLNAPSDW DSRGKDSYET SQLDDQSAET HSHKQSRLYK RKANDESNEH SDVIDSQELS KVSREFHSHE FHSHEDMLVV DPKSKEEDKH LKFRISHELD SASSEVNTRT RPLEQKLISE EDLAANDILD YKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : SPP1 secreted phosphoprotein 1 [ Homo sapiens (human) ]
Official Symbol : SPP1
Synonyms : SPP1; secreted phosphoprotein 1; BNSP, bone sialoprotein I, OPN, osteopontin; osteopontin; BSPI; early T lymphocyte activation 1; ETA 1; uropontin; nephropontin; osteopontin-C; osteopontin-D; SPP1/CALPHA1 fusion; bone sialoprotein 1; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); OPN; BNSP; ETA-1; MGC110940;
Gene ID : 6696
mRNA Refseq : NM_001040060
Protein Refseq : NP_001035149
MIM : 166490
UniProt ID : P10451

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