Recombinant Human SRSF1 Protein (2-248 aa), His-SUMO-tagged

• Cat.No.: SRSF1-809H
• Product Overview: Recombinant Human SRSF1 Protein (2-248 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Transcription. Protein Description: Full Length of Mature Protein.

Specification

• Description: Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 43.6 kDa
• Protein length: 2-248 aa
• AA Sequence: SGGGVIRGPAGNNDCRIYVGNLPPD IRTKDIEDVFYKYGAIRDIDLKNRR GGPPFAFVEFEDPRDAEDAVYGRDG YDYDGYRLRVEFPRSGRGTGRGGGG GGGGGAPRGRYGPPSRRSENRVVVS GLPPSGSWQDLKDHMREAGDVCYAD VYRDGTGVVEFVRKEDMTYAVRKLD NTKFRSHEGETAYIRVKVDGPRSPS YGRSRSRSRSRSRSRSRSNSRSRSY SPRRSRGSPRYSPRHSRSRSRT
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: SRSF1 serine/arginine-rich splicing factor 1 [ Homo sapiens ]
• Official Symbol: SRSF1
• Synonyms: SRSF1; ASF; MGC5228; P33 subunit; SF2; SF2p33; SRp30a; ASF-1; SFRS1; FLJ53078
• Gene ID: 6426
• mRNA Refseq: NM_001078166
• Protein Refseq: NP_001071634
• MIM: 600812
• UniProt ID: Q07955