Recombinant Human SVIP, His-tagged

• Cat.No.: SVIP-140H
• Product Overview: SVIP, 1-77aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-77aa
• Description: Small VCP/p97-interacting protein, also known as SVIP, is involved in a variety of cellular processes, including membrane fusion and ubiquitin-dependent protein degradation. SVIP functions as an inhibitor of the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway. Overexpression of SVIP, on the other hand, increased the levels of p62 protein and enhanced starvation-activated autophagy as well as promoted sequestration of polyubiquitinated proteins and p62 in autophagosomes. Recombinant human SVIP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 2mM DTT
• Molecular Mass: 10.8kDa (100aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGLCFPC PGESAPPTPD LEEKRAKLAE AAERRQKEAA SRGILDVQSV QEKRKKKEKI EKQIATSGPP PEGGLRWTVS
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: SVIP small VCP/p97-interacting protein [ Homo sapiens ]
• Official Symbol: SVIP
• Gene ID: 258010
• mRNA Refseq: NM_148893.1
• Protein Refseq: NP_683691.1
• UniProt ID: Q8NHG7
• Chromosome Location: 11p14.2
• Pathway: Protein processing in endoplasmic reticulum, organism-specific biosystem;Protein processing in endoplasmic reticulum, conserved biosystem